Neurofilament Antibody / Heavy

SKU: orb248480

Description

Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Reactivity: Human, Mouse, Rat
• Application Notes:
  The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.1. The NF421 antibody is specific to the heavy subunit.2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: NF421
• Immunogen: Human recombinant Neurofilament protein was used as the immunogen. (1)
• Purification: Protein G purified Neurofilament antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human cerebellum with Neurofilament antibody (clone NF421).

SDS-PAGE Analysis of Purified, BSA-Free Neurofilament Antibody (clone NF421). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human brain lysate with Neurofilament antibody (clone NF421).

Flow cytometry testing of permeabilized human HEK293 cells with Neurofilament antibody (clone NF421); Red = isotype control, Blue = Neurofilament antibody.

Immunofluorescent staining of permeabilized human HEK293 cells with Neurofilament antibody (clone NF421, green) and Phalloidin (red).

IHC testing of FFPE rat cerebellum with Neurofilament antibody (clone NF421).