NFKB2 Antibody

SKU: orb750602

Description

NFKB2 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Reactivity: Human
• Application Notes:
  This product was assayed by immunoblot and found to be reactive against Human NFKB2 p52 at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti- Human NFKB2 p52 is suitable for the detection by immunoblot of Human NFKB2 p52 and its precursor protein p100. Cross reactivity with p52 from other species may occur but has not been specifically determined. Reactivity in supershift assays has not been determined.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Human NFKB2 p52/p100 peptide corresponding to aa residue 1-19 the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: NFKB2
• Purity: This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-Human NFKB2 p52 may react non-specifically with other proteins. Control peptide (orb343893) will compete only with the specific reaction of antiserum with Human NFKB2 p52.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 90 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit Anti-NFKB p52 antibody, Nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Lyt10, Nuclear factor NF-kappa-B p52 subunit, NFKB2

EMSA results using Anti-NFKB p52. Comparison of nuclear factor (NF)-κB binding activity among CD30-positive lymphomas with or without stimulation of CD30. (a) Whole-cell lysates of CD30-positive lymphoma cell lines with or without stimulation of CD30 were incubated with the 32P-labeled HIV κB probe, and κB binding was analyzed by electrophoretic mobility shift assay (EMSA). (b) Supershift assay of CD30-stimulated Karpas 299 and SR-786 using the antibodies indicated. Addition of anti-Bcl-3 antibodies produced no apparent supershifted bands (data not shown). The specificity of κB binding was determined by competition with an excess amount of unlabeled HIV κB probe or control unlabeled AP1 probe (5'-CGCTTGATGAGTCAGCCGGAA-3'). (c) NF-κB DNA-binding activities of p52-containing components were compared among CD30-positive lymphoma cell lines upon stimulation of CD30. The whole-cell lysates were incubated with the 32P-labeled H2 κB probe, and the binding was analyzed by EMSA. (d) Supershift assay of CD30-stimulated Karpas 299, KM-H2 and KIS-1. The cell lysates were first incubated with antibodies against p50, p52, p65, RelB and c-Rel, and the components of the NF-κB-binding complexes in each cell line were determined. The specificity of κB binding was determined by competition with an excess amount of unlabeled H2 κB probe or control unlabeled AP1 probe.

H1299 cells expressing mutant p53-R175H show increased binding to the NF-κB site. (A) Nuclear extracts of HC-5 and H-R175H were incubated as described in Materials and Methods with a 32P-labeled probe containing the sequence of the NF-κB DNA-binding site. Competition studies were done using a specific competitor (lanes 4 to 7) and a nonspecific competitor (lanes 8 to 11) at both 20× (lanes 4, 5, 8, and 9) and 40× (lanes 6, 7, 10, and 11) molar excess. The single arrows indicate the DNA complexes containing NF-κB complexes. Increased NF-κB activity is observed in the presence of mutant p53 (lanes 2, 3, and 8 to 11). The double arrow indicates the supershifted complex in the presence of antibodies specific for NF-κB1 (p50), NF-κB2 (p52), and p53 (lanes 12 to 15, 20 to 21, and 24 to 29, respectively). Equal amounts of protein were added to each lane.

Quick Database Links

Gene Symbol

NFKB2

UniProt

Q00653

RefSeq (Protein):NP_001070962.1