CYBB Antibody

SKU: orb1239669

Description

CYBB Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2.The immunogen is located within amino acids 140 - 190 of NOX2.
• Target: CYBB
• Molecular Weight: Predicted: 59, 63 kDa Observed: 58 kDa
• Purification: NOX2 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: NOX2 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NOX2 Antibody: CGD, NOX2, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX, Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b558 subunit beta

Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 µg/ml.

Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 µg/ml.

Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 µg/ml.

Immunohistochemistry of NOX2 in rat brain tissue cells with NOX2 antibody at 2 µg/ml.

Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 µg/ml. Green: NOX2 Antibody (orb1239669) Blue: DAPI staining.

Quick Database Links

Gene Symbol

CYBB

UniProt

P04839

RefSeq (Protein):NP_000388