NPAS4 Antibody

SKU: orb376707

Description

Mouse Anti-Rat NPAS4 Monoclonal IgG1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF(1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:100 dilution was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N408/79 (Formerly sold as S408-79)
• Immunogen: Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
• Target: NPAS4
• Molecular Weight: 90kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neuronal PAS domain-containing protein 4, Neuronal PAS4, Class E basic helix-loop-helix protein 79, HLH-PAS transcription factor NXF, PAS domain-containing protein 10, Limbic-enhanced PAS protein, bHLHe79, PASD10, LE-PAS

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone N408/79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.

Western Blot analysis of Rat Brain showing detection of ~90 kDa NPAS4 protein using Mouse Anti-NPAS4 Monoclonal Antibody, Clone N408/79. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band (s): ~60, 45, 40, 38, 25, 20 kDa.

Quick Database Links

Gene Symbol

NPAS4

UniProt

Q8CJH6

NCBI Gene ID

266734

RefSeq (Protein):NP_705890.1