NSE Antibody

SKU: orb376626

Description

Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: NSEL1-1
• Immunogen: Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human pancreas with NSE antibody (clone NSEL1-1). Staining of FFPE tissue requires boiling sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human HeLa cell lysate with NSE antibody (clone NSEL1-1). Predicted molecular weight ~47 kDa.

Quick Database Links

UniProt

P09104