OCLN/Occludin Antibody

SKU: orb1537178

Description

OCLN/Occludin Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Further information: OCLN antibody can be used for detection of OCLN by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 57 kDa Observed: 57 kDa

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A 15 AA synthetic peptide near the C-Terminus of human OCLN. The immunogen is located within the last 50 AA of OCLN.
• Target: OCLN / Occludin
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OCLN, Occludin, BLCPMG

Human Thryoid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence of OCLN in human liver tissue with OCLN antibody at 20 µg/ml.

Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 µg/mL.

Quick Database Links

Gene Symbol

OCLN / Occludin