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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human P selectin / CD62P |
| Target | P-selectin | CD62P |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti P-selectin / CD62P antibody at 1/500 dilution Lysates at 50 µg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294259 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling P-selectin / CD62P with orb1294259 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Cancer tissue labeling P-selectin / CD62P with orb1294259 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Cancer tissue labeling P-selectin / CD62P with orb1294259 at 1/100.

Immunohistochemical analysis of paraffin embedded human tissue labeling P-selectin / CD62P with orb1294259 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded human tissue labeling P-selectin / CD62P with orb1294259 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
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Protocol Information
P-selectin | CD62P Rabbit Polyclonal Antibody (orb1294259)
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