p16INK4a/CDKN2A Antibody

SKU: orb1535834

Description

p16INK4a/CDKN2A Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, KO/KD Validated, WB
• Dilution Range: ELISA, IF (20 µg/ml), IHC, IHC-P (5 µg/ml), WB (1 - 4 µg/ml)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Further information: CDKN2A antibody can be used for detection of CDKN2A by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Western Blot: Predicted: 17 kDa Observed: 20 kDa

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: An 18 amino acid synthetic peptide near the N-Terminus of human CDKN2A. The immunogen is located within the first 50 amino acids of CDKN2A.
• Target: p16INK4a / CDKN2A
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

INK4A, Multiple tumor suppressor 1, MTS-1, MTS1, p16-INK4A, p16 INK4A, p16INK4A, CDKN2A, cyclin-dependent kinase inhibitor 2A

Immunofluorescence of CDKN2A in Human Colon cells with CDKN2A antibody at 20 ug/ml.

Loading: 10 µg of 293 WT cell lysates or KO cell lysates. Antibodies: (2 µg/mL) and beta-actin (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Quick Database Links

Gene Symbol

p16INK4a / CDKN2A