CDKN1A Antibody

SKU: orb1274459

Description

CDKN1A Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IHC, KO/KD Validated, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:20 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human P21 (NP_001207707.1).
• Target: CDKN1A
• Molecular Weight: Observed: 21kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1

Western blot analysis of extracts of U-87MG cells, using P21 antibody (orb1274459) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and P21 knockout (KO) HeLa cells, using P21 antibody (orb1274459) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using P21 antibody (orb1274459) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using P21 antibody (orb1274459) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using P21 antibody (orb1274459) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

CDKN1A

UniProt

P38936