CDKN1B Antibody

SKU: orb345821

Description

p27 kip1 pS140 antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000 - 1:40,000, WB: 1:500 - 1:4,000
• Reactivity: Human
• Application Notes:
  Anti-p27kip1 pS140 antibody is tested for use in ELISA, immunoprecipitation, co-immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. Optimal titers for other applications should be determined by the researcher.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-p27 kip1 pS140 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to S140 residue of human p27 kip1 protein.
• Target: CDKN1B
• Purity: Anti-p27 kip1 pS140 is directed against the phosphorylated form of human p27 kip1 protein at the S140 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. This antibody will specifically react with a p27kip1 protein from human tissue. Reactivity against p27 from other species is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.68 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-p27 kip1 pS140 antibody, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Kip-1, KIP1, CDKN1B

Western Blot of Rabbit anti-p27kip1pS140 Antibody. Lane 1: 293 cells untransfected. Lane 2: 293 cells untransfected and treated. Lane 3: 293 cells transfected with HA-p27wt. Lane 4: 293 cells transfected with HA-p27wt and treated. Lane 5: 293 cells transfected with HA-p27S140A. Lane 6: 293 cells transfected with HA-p27S140A and treated. Load: 20 µg per lane. Primary antibody: p27kip1pS140 antibody at 1:250 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO/TTBS overnight at 4°C. Predicted/Observed size: 27 kDa for p27kip1.

Western Blot of Rabbit anti-p27kip1pS140 antibody. Lane 1-4: 293 pcDNA3.1. Lane 5-8: 293 HA-p27wt. Lane 9-12: 293 HA-p27S140A. Treatment: untreated, 10 µm Etoposide at 5h, 10h, or 24h. Load: 20 µg per lane. Primary antibody: p27kip1pS140 antibody at 1:250 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO/TTBS overnight at 4°C. Predicted/Observed size: 27 kDa for p27kip1pS140.

Quick Database Links

Gene Symbol

CDKN1B

UniProt

P46527

RefSeq:4757962