Paxillin Recombinant Rabbit Monoclonal Antibody

SKU: orb1173108

Description

Paxillin Recombinant Rabbit Monoclonal Antibody

Research Area

Cell Biology, Cytoplasmic, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BG4G0
• Target: PXN
• Molecular Weight: 62 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pax; PAXI_HUMAN; PXN; PAXI_MOUSE; PAXI_RAT; paxillin

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: A431, 3: U87-MG, 4: PC-3, 5: NIH-3T3, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 65 kDa, Observed MW: 45-70 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173108.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line).

Cell line: NIH-3T3, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173108.

Quick Database Links

Gene Symbol

PXN