PCBD1 Rabbit Polyclonal Antibody

SKU: orb312574

Description

PCBD1 Rabbit Polyclonal Antibody

Research Area

Amino Acid Metabolism

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Human, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PCBD1 (2-50/104aa)
• Target: PCBD1
• Molecular Weight: 12 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DCOH; PCD; PHS; PCBD; TCF1; PHS_HUMAN; PCBD1; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha (DCoH | Dimerization cofactor of HNF1); Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase (PCD); 4.2.1.96; PHS_MOUSE; PHS_RAT; pterin-4 alpha-carbinolamine dehydratase 1; 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1); pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1); pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha; Pterin-4a-carbinolamine dehydratase (dimerization cofactor of hepatic nuclear factor 1-alpha); pterin-4-alpha carbinolamine dehydratase; dimerizing cofactor for HNF1

Paraformaldehyde-fixed, paraffin embedded (Mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PCBD1) Polyclonal Antibody, Unconjugated (orb312574) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-PCBD1 (orb312574) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12 kD, Observed band size: 12 kD.

Quick Database Links

Gene Symbol

PCBD1

UniProt

P61457