PDX1 Recombinant Rabbit Monoclonal Antibody

SKU: orb704468

Description

PDX1 Recombinant Rabbit Monoclonal Antibody

Research Area

Cancer Research, Diabetes Research, Insulin and Insulin Like, Insulin and Insulin-like Proteins, Metabolism

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: B56C3
• Immunogen: Recombinant human PDX1 protein
• Target: PDX1
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IPF1; PDX-1; GSF; IDX-1; IUF1; MODY4; PAGEN1; STF-1; IPF-1; Idx1; Stf1; PDX1_HUMAN; PDX1; Glucose-sensitive factor (GSF); Insulin promoter factor 1 (IPF-1); Insulin upstream factor 1 (IUF-1); Islet/duodenum homeobox-1 (IDX-1); Somatostatin-transactivating factor 1 (STF-1); PDX1_MOUSE; Islet/duodenum homeobox 1 (IDX-1); PDX1_RAT; pancreatic and duodenal homeobox 1; insulin promoter factor 1, homeodomain transcription factor; Islet/duodenum homeobox-1; somatostatin transcription factor 1; Glucose-sensitive factor; insulin upstream factor 1

Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PDX1) Monoclonal Antibody, Unconjugated (orb704468) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: ASPC-1 (Human) Cell Lysate at 30 ug, Lane 2: Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-PDX1 (orb704468) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 45 kD.

Quick Database Links

Gene Symbol

PDX1

UniProt

P52945