PEF1 Rabbit Polyclonal Antibody

SKU: orb101553

Description

PEF1 Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Monkey, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PEF1 (121-230/284aa)
• Target: PEF1
• Molecular Weight: 30 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ABP32; PEF1A; 2600002E23Rik; Peflin; PEF1_HUMAN; PEF1; PEF protein with a long N-terminal hydrophobic domain; Penta-EF hand domain-containing protein 1; PEF1_MOUSE; PEF1_RAT; penta-EF-hand domain containing 1

Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA (green).

Sample: Intestine (Mouse) Lysate at 40 ug, Primary: Anti-PEF1 (orb101553) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.

Quick Database Links

Gene Symbol

PEF1

UniProt

Q9UBV8