PRF1 Antibody

SKU: orb1261930

Description

PRF1 Antibody

Research Area

Cell Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_001076585.1).
• Target: PRF1
• Molecular Weight: Observed: 70kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PFP, Perforin-1, Cytolysin, P1, PFP, FLH2, PFN1, HPLH2

Western blot analysis of extracts of various cell lines, using Perforin antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded mouse spleen using Perforin antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using Perforin antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Perforin antibody. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

PRF1

UniProt

P14222