Peroxiredoxin 1 Recombinant Rabbit Monoclonal Antibody

SKU: orb544576

Description

Peroxiredoxin 1 Recombinant Rabbit Monoclonal Antibody

Research Area

Inflammation, Inflammatory Mediators, Oxidative Stress

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: B9C3
• Immunogen: Recombinant human Peroxiredoxin 1 protein, full length
• Target: PRDX1
• Molecular Weight: 22 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRX1; MSP23; NKEF-A; NKEFA; PAG; PAGA; PAGB; PRXI; TDPX2; OSF-3; OSF3; PrdxI; TDX2; TPxA; Hbp23; PRDX1_CRIGR; PRDX1; Thioredoxin peroxidase 2 (TPX-2); Thioredoxin-dependent peroxiredoxin 1; 1.11.1.24; PRDX1_HUMAN; Natural killer cell-enhancing factor A (NKEF-A); Proliferation-associated gene protein (PAG); Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; PRDX1_MOUSE; Macrophage 23 kDa stress protein; Osteoblast-specific factor 3 (OSF-3); PRDX1_RAT; Heme-binding 23 kDa protein; peroxiredoxin 1

Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Peroxiredoxin 1) Polyclonal Antibody, Unconjugated (orb544576) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, U2OS (Human) Cell Lysate at 30 ug, Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Peroxiredoxin 1 (orb544576) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Quick Database Links

Gene Symbol

PRDX1

UniProt

Q06830