PHB2 Recombinant Rabbit Monoclonal Antibody

SKU: orb1172474

Description

PHB2 Recombinant Rabbit Monoclonal Antibody

Research Area

Coactivators and Corepressors, DNA and RNA Research, Epigenetics and Nuclear Signaling, Hormone Research, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200, IHC-P=1:100-200, IHC-F=1:100-200, IF=1:100-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG/Kappa
• Clone No.: BH7I0
• Target: PHB2
• Molecular Weight: 33 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BAP; BCAP37; Bap37; PNAS-141; REA; hBAP; p22; Bap-37; Bcap27; PHB2_HUMAN; PHB2; B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity; PHB2_MOUSE; PHB2_RAT; B-cell receptor-associated protein BAP37 (BAP-37); prohibitin 2; B cell receptor associated protein 37

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:10000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HeLa, 293, Mouse heart, Rat kidney, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 33 kDa, Observed MW: 33 kDa.

Cell line: HeLa, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172474.

Quick Database Links

Gene Symbol

PHB2