PGF Antibody

SKU: orb1272744

Description

PGF Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Direct:To detect hPlGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.SandwichTo detect hPlGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human PlGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF. Western Blot:To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPlGF. Human PlGF specific antibody was purified by affinity chromatography employing immobilized hPlGF matrix.
• Target: PGF
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PGFL, PLGF, PlGF-2, D12S1900, SHGC-10760, PGFL, Placenta growth factorPlGF

To detect hPlGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PlGF (orb1272743) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.

To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.0 ug/ml with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

PGF

UniProt

P49763

RefSeq:P49763