PNPLA2/ATGL Antibody

SKU: orb19789

This product can be custom made

Description

Goat polyclonal antibody to PNPLA2

Research Area

Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IF
Dilution Range	ELISA: 1:8000, WB: 0.2-0.6 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 55kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.2-0.6 μg/ml.

Key Properties

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Clonality	Polyclonal
Target	PNPLA2 / ATGL
Protein Sequence	PARPHGPEDKDQ
Molecular Weight	55.3
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti PNPLA2 antibody, anti ATGL antibody, anti patatin-like phospholipase domain containing 2 antibody, anti 1110001C14Rik antibody, anti DKFZp667M109 antibody, anti FP17548 antibody, anti TTS-2.2 antibody, anti adipose triglyceride lipase antibody, anti desnutrin antibody, anti transport-secretion protein 2.2 antibody, anti triglyceride hydrolase antibody

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Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing lipid droplet and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

PNPLA2 / ATGL

NCBI Gene ID

57104

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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