PRNP Antibody

SKU: orb1258104

Description

PRNP Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human PRNP (NP_001073592.1).
• Target: PRNP
• Molecular Weight: Observed: 20-30kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRNP, prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia), ASCR, CD230, CJD, GSS, MGC26679, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, CD230 antigen, major prion protein, prion protein, prion protein (p27-30) (Creutzfeld-Jakob disease Gerstmann-Strausler-Scheinker syndrome, prion protein PrP, prion-related protein , KURU, AltPrP, p27-30

Western blot analysis of extracts of mouse brain, using PRNP antibody (orb1258104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat heart using PRNP Antibody (orb1258104) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human smooth muscle using PRNP Antibody (orb1258104) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

PRNP

UniProt

P04156