Rab5 Antibody

SKU: orb153348

Description

This goat polyclonal antibody detects mouse Rab5, a Rab-family small GTPase that serves as an early endosome marker and regulates vesicular trafficking at the onset of endocytosis.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P
• Dilution Range: WB:1:250-1:2,000, IF:1:50-1:250, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000
• Reactivity: Canine, Human, Monkey, Mouse, Rat
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a, Rab5b and rab5c produced in E. coli.
• Target: RAB5A, member RAS oncogene family
• Purification: Epitope affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: ​Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAB5A, RAB5B, RAB5C, RAS-associated protein RAB5A, ras-related protein Rab-5B, RAS-associated protein RAB5C, ras-related protein Rab-5A, ras-related protein Rab-5B, ras-related protein Rab-5C antibody.

IF – Rab5 Antibody using B6-RPE07 cells at 1/50 dilution; cells were fixed with 4% of PFA.

Rab5 antibody at 1/1,000 dilution; tissue lysate at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.

IF – Rab5 Antibody using B6-RPE07 cells at 1/50 dilution; cells were fixed with 4% of PFA.

IF – Rab5 Antibody using B6-RPE07 cells at 1/50 dilution; cells were fixed with 4% of PFA.

IHC of human gallbladder using Rab5 antibody and FFPE tissue after heat-induced antigen retrieval. Rab5 Ab at 1:100/DAB detection.

IHC of mouse stomach using Rab5 antibody and FFPE tissue after heat-induced antigen retrieval. Rab5 Ab at 1:500/DAB detection.

IHC of human myocardium using Rab5 antibody and FFPE tissue after heat-induced antigen retrieval. Rab5 Ab at 1:100/DAB detection.

Quick Database Links

Gene Symbol

RAB5A, member RAS oncogene family