Rabbit B7-H3/CD276 Antibody

SKU: orb1523124

Description

B7-H3/CD276 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. Use rabbit anti-B7-H3/CD276 antibody A304-367A.; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 484 and C-terminus
• Target: B7-H3/CD276
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4Ig-B7-H3; B7 homolog 3; B7H3; B7-H3; B7RP-2; CD antigen CD276; CD276 antigen; costimulatory molecule

Detection of human B7-H3/CD276 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-B7-H3/CD276 antibody orb1523124 used for IP at 6 µg per reaction.

Detection of human B7-H3/CD276 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. orb1523124) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB Counterstain: IHC hematoxylin

Detection of human B7-H3/CD276 by immunocytochemistry. Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. orb1523124) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

B7-H3/CD276

UniProt

Q5ZPR3

RefSeq (Protein):NP_001019907.1