Rabbit CAR Antibody

SKU: orb1525887

Description

CAR Antibody

Research Area

Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 315 and C-terminus
• Target: CAR
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

46 kD coxsackievirus and adenovirus receptor (CAR) protein; CAR; CAR4/6; coxsackie virus and adenovirus receptor; coxsackievirus and adenovirus receptor; coxsackievirus B-adenovirus receptor; CVB3-binding protein; HCAR; HCVADR

Detection of human CAR by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CAR antibody orb1525887 used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. CAR was also immunoprecipitated by rabbit anti-CAR antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human CAR by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CAR (Cat. No. orb1525887) used at a dilution of 1: 1, 000 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate

Quick Database Links

Gene Symbol

CAR

UniProt

P78310

RefSeq (Protein):NP_001329.1