Rabbit DEAF1 Antibody

SKU: orb1525296

Description

DEAF1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 515 and C-terminus
• Target: DEAF1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

deformed epidermal autoregulatory factor 1 homolog; MRD24; nuclear DEAF-1-related transcriptional regulator; NUDR; SPN; suppressin; zinc finger MYND domain-containing protein 5; ZMYND5

Detection of human DEAF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DEAF1 antibody orb1525296 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. DEAF1 was also immunoprecipitated by rabbit anti-DEAF1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).

Quick Database Links

Gene Symbol

DEAF1

UniProt

O75398

RefSeq (Protein):NP_066288.2