Rabbit KAP-1 Antibody

SKU: orb1530436

Description

KAP-1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:500 - 1:2,500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: KAP-1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB Kruppel-associated box domain-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; TIF1B; TIF1-beta; transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; Tripartite motif-containing protein 28

Detection of mouse KAP-1 by western blot.Samples: Whole cell lysate from TCMK-1 and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-KAP-1 antibody orb1530436 at 0.04 µg/ml.

Detection of human KAP-1 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-KAP-1 antibody orb1530436 at 0.04 µg/ml.

Detection of human KAP-1 by immunohistochemistry.Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-KAP-1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human KAP-1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody.

Quick Database Links

Gene Symbol

KAP-1

UniProt

Q13263

RefSeq (Protein):NP_005753.1