Rabbit Myc Tag Antibody

SKU: orb1530878

Description

Myc Tag Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IP, WB
• Dilution Range: WB - 1:1,000 - 1:30,000; IP - 1 - 4 µg/mg lysate; ICC - 1:100 - 1:400; ELISA - 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
• Application Notes:
  Format: Whole IgG. Application Information: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Target: Myc Tag
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MYCC; v-myc avian myelocytomatosis viral oncogene homolog; transcription factor p64; v-myc myelocytomatosis viral oncogene homolog; myc-related translation/localization regulatory factor; MRTL; c-Myc; class E basic helix-loop-helix protein 39; bHLHe39; avian myelocytomatosis viral oncogene homolog; proto-oncogene c-Myc; myc proto-oncogene protein

Detection of c-myc-tagged protein by western blot.Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified rabbit anti-Myc Tag antibody orb1530878 at 0.04 µg/ml (1:25, 000).

Detection of c-myc-tagged Plakoglobin by immunofluorescence.Samples: Human microvascular endothelial cells expressing c-myc tagged plakoglobin following transient transfection. Antibody: Affinity purified rabbit anti-Myc Tag used at 1 µg/ml.

Quick Database Links

Gene Symbol

Myc Tag

UniProt

P01106