Rabbit SAE1 Antibody

SKU: orb1525753

Description

SAE1 Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 296 and C-terminus
• Target: SAE1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

activator of SUMO1; AOS1; HSPC140; sentrin/SUMO-activating protein AOS1; SUA1; SUMO-1 activating enzyme E1 N subunit; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; UBLE1A

Detection of human SAE1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SAE1 antibody orb1525753 used for IP at 3 µg per reaction.

Detection of human SAE1 by immunohistochemistry.Sample: FFPE section of human bladder carcinoma. Antibody: Affinity purified rabbit anti-SAE1 (Cat. No. orb1525753) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SAE1

UniProt

Q9UBE0

RefSeq (Protein):NP_005491.1