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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:50 - 1:250 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 2422 and C-terminus |
| Target | SPTAN1/Alpha II-spectrin |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human and mouse SPTAN1/Alpha II-Spectrin by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-SPTAN1/Alpha II-Spectrin antibody orb1528758 at 0.04 µg/ml

Detection of human SPTAN1/Alpha II-Spectrin by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SPTAN1/Alpha II-Spectrin used at a dilution of 1:200 (1µg/ml).

IHC: Sample: human breast carcinoma. Antibody: Affinity purified rabbit anti-SPTAN1/Alpha II-Spectrin at 1:200. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003118.1 |
|---|
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Protocol Information
Rabbit SPTAN1/Alpha II-spectrin Antibody (orb1528758)
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