rAra h 2 (Arachis hypogaea 2.0201) Antibody

SKU: orb1088513

Description

rAra h 2 (Arachis hypogaea 2.0201) Antibody

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Application Notes:
  Recombinant protein rAra h 2 is expressed in Escherichia coli. DNA sequence encoding 164 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,5 kDa.

Key Properties

• Purification: Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Recombinant allergen rAra h 2 purity verification. 5 µg of rAra h 2 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

Presence of sIgE antibodies specific to recombinant allergens Ara h 1, Ara h 2, and Ara h 8 was analysed in plasma of a patient with confirmed presence (routine clinical test) of specific IgE antibodies to peanut allergen extract. Western blotting analysis was performed on recombinant allergens rAra h 1, rAra h 2, and rAra h 8 mixed and heated (100°C, 5 min) with reducing SDS loading buffer. Samples were resolved using 15% Tris-glycine gel electrophoresis. Nitrocellulose membrane blot was incubated with plasma of an allergic patient (1:10). Captured sIgE antibodies were detected by biotinylated anti-human IgE secondary antibody (2 μg/ml) followed by IRDye 800CW Streptavidin (1:5000). The analysed patient plasma contained sIgE antibodies specific to Ara h 2.

Quick Database Links

UniProt

Q6PSU2

NCBI Gene ID

35