Rat IgG2a Isotype Control Monoclonal Antibody (PE)

SKU: orb1293831

Description

The Rat IgG2a Isotype Control-PE labeled Antibody is suitable for FC, IF. It is a Monoclonal, PE conjugated antibody. Purification: The antibody was purified by affinity chromatography.

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: 10 μl / assay
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone No.: B4C5
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Shipped and store at 4°C for one year. Do not freeze.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only