KRT7 Antibody

SKU: orb1274234

Description

KRT7 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG, kappa
• Clone No.: KRT7/1499R
• Immunogen: Full-length human CK7 protein was used as the immunogen for the recombinant Cytokeratin 7 antibody.
• Target: KRT7
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL

IHC testing of FFPE human oviduct with recombinant Cytokeratin 7 antibody (clone KRT7/1499R). Required HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Western blot testing of human HeLa cell lysate with recombinant Cytokeratin 7 antibody (clone KRT7/1499R).

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytokeratin 7 antibody (clone KRT7/1499R); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 7 antibody (clone KRT7/1499R) as confirmation of integrity and purity.

Quick Database Links

Gene Symbol

KRT7

UniProt

P08729