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Recombinant Human Sonic hedgehog protein(SHH) (Active)
Description
Research Area
Images & Validation
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Key Properties
−| Expression System | E.Coli |
|---|---|
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H,10T1,2 cells is less than 1 g,ml, corresponding to a specific activity of 1.0 103 IU,mg. |
| Tag | NO-tagged |
| MW | 19.8 kDa |
| Purity | >98% as determined by SDS-PAGE and HPLC. |
| Protein Sequence | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
|---|---|
| Form/Appearance | 0.2 ?m filtered 20 mM PB, pH 7.4, 150mM NaCl ,lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−RecombinantShh,Mouse(CHO-expressed) [orb1494667]
> 95% as analyzed by SDS-PAGE and HPLC.
20 kDa, observed by non-reducing SDS-PAGE.
CHO
Recombinant Human Sonic HedgeHog (Shh) [orb1494940]
>98% by SDS-PAGE and HPLC analyses.
Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
Escherichia coli

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Protocol Information
Recombinant Human Sonic hedgehog protein(SHH) (Active) (orb1650543)
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