RecombinantIL-3,Human

SKU: orb1494821

Description

Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific α chain and a common β chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family. Recombinant human Interleukin-3 (rhIL-3) produced in E.coli is a single non-glycosylated polypeptide chain containing of 134 amino acids. A fully biologically active molecule, rhIL-3 has a molecular mass of 15.2 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/mL.

Key Properties

• Source: Escherichia coli.
• Biological Activity: ED50 2 × 10ˆ7 units/mg.
• Purification: > 95% as analyzed by SDS-PAGE and HPLC.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• MW: 15.2 kDa, observed by non-reducing SDS-PAGE.
• Purity: > 95% as analyzed by SDS-PAGE and HPLC.
• Protein Sequence: MAPMTQTTP LKTSWVNCSN MIDEIITHLK QPPLPLLDFN NLNGEDQDIL MENNLRRPNL EAFNRAVKSL QNASAIESIL KNLLPCLPLA TAAPTRHPIH IKDGDWNEFR RKLTFYLKTL ENAQAQQTTL SLAIF

Storage & Handling

• Storage: Lyophilized recombinant human Interleukin-3 (rhIL-3) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-3 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-3, IL3