RecombinantIL-6,Human

SKU: orb1494814

Description

Interleukin-6 (IL-6, also known as IFN-β2) is a pleiotropic cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 is secreted by T cells and macrophages to stimulate immune response. IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells.Recombinant human Interleukin-6 (rhIL-6) produced in E.coli is a single non-glycosylated polypeptide chain containing 185 amino acids. A fully biologically active molecule, rhIL-6 has a molecular mass of 20.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/ml.

Key Properties

• Source: Escherichia coli.
• Biological Activity: ED50 1 x 10ˆ7 units/mg.
• Purification: > 95% by SDS-PAGE analysis.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• MW: 20.9 kDa, observed by reducing SDS-PAGE.
• Purity: > 95% by SDS-PAGE analysis.
• Protein Sequence: MPVPPGEDS KDVAAPHRQP LTSSERIDKQ IRYILDGISA LRKETCNKSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEETCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PDPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSSL RALRQM

Storage & Handling

• Storage: Lyophilized recombinant human Interleukin-6 (rhIL-6) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-6 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against 1xPBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 1xPBS.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-6, IL6