RHOT1 Antibody

SKU: orb1239136

Description

RHOT1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus
• Application Notes:
  RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1.The immunogen is located within amino acids 40 - 90 of RHOT1.
• Target: RHOT1
• Molecular Weight: Predicted: 76 kDa Observed: 78 kDa
• Purification: RHOT1 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: RHOT1 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ras homolog family member T1, mitochondrial Rho GTPase 1, ARHT1, MIRO-1, MIRO1, MIRO

Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 µg/ml.

Immunohistochemistry of RHOT in mouse brain tissue with RHOT antibody at 5 µg/ml.

Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 µg/ml.

Quick Database Links

Gene Symbol

RHOT1

UniProt

Q8IXI2

RefSeq (Protein):NP_001028740