RNF139/TRC8 Antibody

SKU: orb1539139

Description

RNF139/TRC8 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.3 - 1 µg/ml)
• Reactivity: Human, Monkey
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Experiments gave bands at approx. 150kDa and 50kDa in Human Skeletal Mucle lysate after 0.5 ug/ml antibody staining. This protein has a calculated MW of 76.0kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 ug/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-HTGAAAEEFNDDT, from the C-Terminus of protein sequence according to NP_009149.2.
• Target: RNF139 / TRC8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RNF139, HRCA1, RCA1, TRC8, Ring finger protein 139

Goat Anti-TRC8 Antibody (1 µg/ml) staining of Human Skeletal Muscle lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.

Quick Database Links

Gene Symbol

RNF139 / TRC8