ROCK1 Antibody

SKU: orb1261296

Description

ROCK1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: [H5E4]
• Immunogen: Recombinant protein of human ROCK1
• Target: ROCK1
• Molecular Weight: Observed: 158kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, ROCK-I

Western blot analysis of extracts of various cell lines, using ROCK1 antibody (orb1261296). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse thyroid using ROCK1 antibody (orb1261296) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid using ROCK1 antibody (orb1261296) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using ROCK1 antibody (orb1261296) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

ROCK1

UniProt

Q13464