S100B Mouse Monoclonal Antibody

SKU: orb500644

Description

S100B Mouse Monoclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B3E8
• Immunogen: Recombinant human S100B protein (2-92/92aa (N-6x His-Tag)aa)
• Target: S100B
• Molecular Weight: 10 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEF; S100; S100-B; S100beta; S100B_HUMAN; S100B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100 calcium binding protein B; S100 calcium binding protein, beta (neural)

(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human glioblastoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of orb500644 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Sample: Lane 1: Cerebral cortex (Rat) Lysate at 40 ug, Lane 2: Spinal cord (Mouse) Lysate at 40 ug, Lane 3: Spinal cord (Rat) Lysate at 40 ug, Lane 4: Cerebellum (Mouse) Lysate at 40 ug, Lane 5: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-S100B (orb500644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 11 kD, Observed band size: 9 kD.

Quick Database Links

Gene Symbol

S100B

UniProt

P04271