SARS-CoV-2 (COVID-19) nucleocapsid Rabbit Polyclonal Antibody

SKU: orb1294283

Description

Rabbit polyclonal antibody to SARS-CoV 2 nucleocapsid protein

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: IF, NeA, WB
• Dilution Range: Western Blot 1:3000-1:5000, Immunofluorescence 1:500-1:1000
• Reactivity: Virus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Recombinant fragment corresponding to SARS-CoV 2 nucleocapsid protein
• Target: SARS-CoV-2 (COVID-19) nucleocapsid
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: anti-SARS-CoV-2 nucleocapsid protein antibody at 1/3000 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294283 overnight at 4°C.

Immunofluorescence: Cells were stained with orb1294283 anti-SARS-CoV-2 nucleocapsid protein antibody (green) at 1:500 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

SARS-CoV-2 (COVID-19) nucleocapsid