SARS S Protein, His Tag

SKU: orb750333

Description

SARS S protein (R667A, K968P, V969P), His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Research Area

Infectious Disease & Virology

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: Spike protein
• Reactivity: Virus
• Tag: C-10×His
• Expression Region: Ser 14 - Pro 1195
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 136.3 kDa
• Purity: 95%

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, 0.2 M Sodium citrate, pH 5.5
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Spike protein

UniProt

P13567

RefSeq:R667, K968, V969, AAP13567.1