Semaphorin 3F Rabbit Polyclonal Antibody

SKU: orb11360

Description

Semaphorin 3F Rabbit Polyclonal Antibody

Research Area

Axonal Guidance Proteins, Neurogenesis, Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Sema3F (651-754/754aa)
• Target: SEMA3F
• Molecular Weight: 88 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SEMA-IV; SEMA4; SEMAK; SemaIV; SEM3F_HUMAN; SEMA3F; Sema III/F; Semaphorin IV (Sema IV); SEM3F_MOUSE; semaphorin 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema IV

Cell culture (A549), Paraformaldehyde-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (semaphorin 3F) Polyclonal Antibody, Unconjugated (orb11360) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining.

Cell culture (U251), Paraformaldehyde-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (semaphorin 3F) Polyclonal Antibody, Unconjugated (orb11360) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Semaphorin 3F) Polyclonal Antibody, Unconjugated (orb11360) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (semaphorin 3F) Polyclonal Antibody, Unconjugated (orb11360) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Sema3F Polyclonal Antibody, Unconjugated (orb11360) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SEMA3F

UniProt

Q13275