Sheep Rat Serum proteins Antibody

SKU: orb22451

Description

Precipitating polyclonal rabbit antiserum to swine serum proteins. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in cat milk or other body fluids or to determine its concentration. To prepare an immune adsorbent for the purification of cat IgA from serum or exocrine secretions. Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.

Images & Validation

• Tested Applications: DOT, IEP
• Reactivity: Rat
• Application Notes:
  Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross-reactivity. Consequently it is not species- specific.IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.

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Key Properties

• Antibody Type: Secondary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added.
• Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only