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Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC, IHC-P, WB |
|---|---|
| Dilution Range | IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:128000), WB (0.03 - 0.3 µg/ml) |
| Reactivity | Human, Monkey |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Peptide with sequence C-KGQTQNFKDAFSGRD, from the internal region of the protein sequence according to NP_055146.1. |
| Target | SLC7A11 / XCT |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
| Concentration | 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Goat Anti-SLC7A11 Antibody Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Goat Anti-SLC7A11 Antibody (0.3 µg/ml) staining of A549 (A) and U2OS (B) cell ysate (35 µg protein in RIPA buffer). Detected by chemiluminescencence.

Goat Anti-SLC7A11 Antibody Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton.
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Protocol Information
SLC7A11/XCT Antibody (orb1538307)
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