SMAD1 Rabbit Polyclonal Antibody

SKU: orb500665

Description

SMAD1 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Gallus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SMAD1 (151-250/465aa)
• Target: SMAD1
• Molecular Weight: 51 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mad1; Mlp1; MusMLP; dwf-A; mMad1; SMAD1_HUMAN; SMAD1; MAD homolog 1; Mothers against DPP homolog 1; Mad-related protein 1; SMAD family member 1 (SMAD 1 | Smad1 | hSMAD1); Transforming growth factor-beta-signaling protein 1 (BSP-1); SMAD1_MOUSE; Dwarfin-A (Dwf-A); Mothers-against-DPP-related 1 (Mad-related protein 1 | mMad1); SMAD family member 1 (SMAD 1 | Smad1); SMAD1_RAT; SMAD family member 1; MAD, mothers against decapentaplegic homolog 1 (Drosophila); SMAD, mothers against DPP homolog 1 (Drosophila)

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD1) Polyclonal Antibody, Unconjugated (orb500665) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD1) Polyclonal Antibody, Unconjugated (orb500665) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: H9C2 (Rat) Cell Lysate at 30 ug, Primary: Anti-SMAD1 (orb500665) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Sample: Heart (Mouse) Lysate at 40 ug, Muscle (Mouse) Lysate at 40 ug, Primary: Anti-SMAD1 (orb500665) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-SMAD1 (orb500665) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Tissue/Cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MDFI Polyclonal Antibody, Unconjugated (orb500665) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SMAD1

UniProt

Q15797