SOX2 Antibody

SKU: orb1240008

Description

SOX2 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Gallus, Porcine, Sheep
• Application Notes:
  SOX2 antibody can be used for detection of SOX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: SOX2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SOX.The immunogen is located within the first 50 amino acids of SOX2.
• Target: SOX2
• Purification: SOX2 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: SOX2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2

Western blot analysis of SOX2 in 3T3 cell lysate with SOX2 antibody at (A) 1 and (B) 2 µg/mL.

Immunocytochemistry of SOX2 in 3T3 cells with SOX2 antibody at 5 µg/mL.

Immunofluorescence of SOX2 in 3T3 cells with SOX2 antibody at 20 µg/mL.

Immunohistochemistry of SOX2 in mouse brain tissue with SOX2 Antibody at 5 µg/mL.

Immunofluorescence of SOX2 in mouse brain tissue with SOX2 Antibody at 20 µg/mL. Green: SOX2 antibody (orb1240008) Red: Phylloidin staining Blue: DAPI staining.

Quick Database Links

Gene Symbol

SOX2

UniProt

P48431

RefSeq (Protein):NP_003097