SPP1/Osteopontin Antibody

SKU: orb1537794

Description

SPP1/Osteopontin Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.1 - 0.3 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-DPKSKEEDKHLK, from the C-Terminus of protein sequence according to NP_001035147.1NP_000573.1NP_001035149.1.
• Target: SPP1 / Osteopontin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I

SPP1 / Osteopontin antibody (0.5 µg/ml) staining of MOLT4 lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SPP1 / Osteopontin