SUPT16H/FACTP140 Antibody

SKU: orb1537701

Description

SUPT16H/FACTP140 Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (10 µg/ml), WB
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 8D2
• Immunogen: Recombinant human SUPT16H (full length).
• Target: SUPT16H / FACTP140
• Purification: Affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. The.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SUPT16H, CDC68, FACT140, FACT, FACT complex subunit SPT16, FACT 140 kDa subunit, HSPT16, SPT16/CDC68, FACTP140

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Spt16 (Clone 8D2) antibody.

Quick Database Links

Gene Symbol

SUPT16H / FACTP140