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SYNPO/Synaptopodin Antibody

SKU: orb1538549

Description

SYNPO/Synaptopodin Antibody

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, IF, IHC, IHC-P, WB
Dilution RangeELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
ReactivityHuman, Mouse, Rat
Application Notes
Further information: SYNPO antibody can be used for detection of SYNPO by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 75, 99, 102 kDa Observed: 95 kDa

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenA 17 AA synthetic peptide near the C-Terminus of human SYNPO. The immunogen is located within the last 50 AA of SYNPO.
TargetSYNPO / Synaptopodin
PurificationPeptide Column Affinity Chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS containing 0.02% Sodium Azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SYNPO, KIAA1029, Synaptopodin

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SYNPO/Synaptopodin Antibody

Immunofluorescence of SYNPO in human brain tissue with SYNPO antibody at 20 ug/ml.

SYNPO/Synaptopodin Antibody

Western blot analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 ug/ml.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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SYNPO/Synaptopodin Antibody (orb1538549)

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0.05 mg
$ 810.00
Free Secondary Antibody (20 ul)0/0

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