Tau Antibody (HRP)

SKU: orb536193

Description

Rabbit polyclonal antibody against Tau conjugated to HRP

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:1000 dilution was sufficient for detection of Tau in 10 μg of human SH-SY5Y cell lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Human Tau 2NR4 P301S Fibril
• Target: Tau
• Molecular Weight: 45.8kDa
• Purification: Protein A Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tau, TAU, TAU_HUMAN, MAPT, MAPTL, Microtubule-associated protein tau, Microtubule associated protein tau, Microtubule associated protein tau isoform 4, Neurofibrillary tangle protein, Paired helical filament tau, Paired helical filament-tau, PHF tau, PHF-tau, DDPAC, FTDP 17, G protein beta1/gamma2 subunit interacting factor 1, MSTD, Mtapt, MTBT1, MTBT2, PPND, PPP1R103, Protein phosphatase 1 regulatory subunit 103, RNPTAU, AI413597, AW045860, FLJ31424, MGC134287, MGC138549, MGC156665

Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Brain Cortex. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody.

Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band (s): 50 kDa.

Quick Database Links

Gene Symbol

Tau

UniProt

P10636

NCBI Gene ID

4137