TYMS Antibody

SKU: orb345029

Description

Thymidylate Synthase antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  THYMIDYLATE SYNTHASE antibody has been tested by western blot. This antibody is suitable for use in ELISA, immunoprecipitation, immunofluorescence microscopy, and immunohistochemistry. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-THYMIDYLATE SYNTHASE was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
• Target: TYMS
• Purity: Anti-THYMIDYLATE SYNTHASE IgG fraction is directed against human Thymidylate Synthase and is useful in determining its presence in various assays. Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

sheep anti-Thymidylate Synthase Antibody, d TMP synthase antibody, EC 2.1.1.45 antibody, HsT422 antibody, MGC88736 antibody

Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2000, 1:5000 and 1:10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (orb347924) diluted 1:1000 and color development using TMB (orb348656) substrate for approximately 4'.

Western Blot results of Sheep anti-Thymidylate Synthase Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: HeLa WCL (p/n orb348668). Lane 3: MOLT-4 WCL (p/n orb348696). Load: 10 µg/lane. Primary Antibody: Sheep anti-Thymidylate Synthase at 1 µg/ml overnight at 4°C. Secondary Antibody: Donkey anti-Sheep HRP (p/n orb347953) at 1:40000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT. Predicted MW: ~36kDa. Observed MW: ~35kDa.

Quick Database Links

Gene Symbol

TYMS

UniProt

P04818

RefSeq:P04818.3